Lymph node
- Section of a portion of the outer cortex of a lymph node
- showing the capsule, subcapsular sinuses, diffuse lymphoid tissue, and lymphatic nodules
- H & E stain
- Medium magnification
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Lymph node: sections
Figure A
- Section of a lymph node showing the cortex and the medulla & their primary components
- H & E stain
- Low magnification
Figure B
- (1) capsule
- (2) lymphoid nodule with germinative center
- (3) subcapsular sinus
- (4) intermediate sinus
- (5) medullary cords
- (6) medullary sinus
- (7) trabecula
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Lymph node: Medulla
Figure A
- Medullary cord of lymphoid nodule
- In addition to lymphocytes, this area contains many plasma cells (ARROWS)
Figure B
- Label 1: Medullary sinus
- Label 2: Medullary cord
- H & E Stain
- High magnification
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Lymph node: Medulla
Figure A
- A photomicrograph of the medulla of a lymph node
- The medullary sinuses are separated by medullary cords
- Lymphocytes clearly predominate in number over other cell types
- A blood vessel within a medullary cord is also seen
- H & E Stain
- Medium magnification
Figure B
- (1) Medullary cords
- (2) Medullary sinuses
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Lymphoid nodule of the spleen
- Lymphoid nodule of the spleen
- Surrounded by red pulp
- Label 1: A germinating center
- Label 2: The (eccentric) central artery
- Which are characteristics of the spleen & are clearly visible
- 2 small sections of sheathed arteries are seen to the right of the nodule
- H & E stain
- Medium magnification
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Bone marrow: normal
- This is the appearance of a normal bone marrow
- Note the presence of:
- megakaryocytes
- erythroid islands
- granulocytic precursors
- high magnification
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Bone marrow smear: Normal
- This is the appearance of a normal bone marrow smear
- Note the presence of:
- megakaryocytes
- erythroid precursors
- granulocytic precursors
- High magnification
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Spleen: Section
- The capsule is seen sending the trabeculae to the interior of the organ
- The red pulp occupies most of the microscopic field
- Note the white pulp with its arterioles
- Pirosirius stain
- Low magnification
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Spleen: Section
Figure A
- Section of spleen showing the red pulp (most of microscopic field) and a component of the white pulp – a periarterial lymphatic sheath (PALS) surrounding a central artery
- Small sections of trabeculae are present in the red pulp
- H & E stain
- Low magnification
Figure B
- (1) PALS
- (2) central artery
- (3) red pulp
- (4) sinusoids
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Blood smear
- The RBCs here are normal
- Have a zone of central pallor about 1/3 size of the RBC
- The RBCs demonstrate minimal variation is size (anisocytosis) and shape (poikilocytosis)
- In the center of the field: A band of neutrophil (LEFT) and segmented n
eutrophil (RIGHT)
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Blood smear
- In the center of the field: eosinophil with a bilobed nucleus & numerous reddish granules in the cytoplasm.
- Just underneath eosinophil: small lymphocyte
- Eosinophils can increase with: allergic reactions & parasitic infestations
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Blood smear
- In the center of the field: basophil with a lobed nucleus (like PMNs) & numerous coarse, dark blue granules in the cytoplasm
- Infrequent in a normal peripheral blood smear
- Significance is uncertain
- Left: Band neutrophil
- Right: Large activated lymphocyte