Haematology FLMs

  • By: Terri
  • Date: October 7, 2009
  • Time to read: 2 min.

Lymph node

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  • Section of a portion of the outer cortex of a lymph node
  • showing the capsule, subcapsular sinuses, diffuse lymphoid tissue, and lymphatic nodules
  • H & E stain
  • Medium magnification

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Lymph node: sections

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Figure A

  • Section of a lymph node showing the cortex and the medulla & their primary components
  • H & E stain
  • Low magnification

Figure B

  • (1) capsule
  • (2) lymphoid nodule with germinative center
  • (3) subcapsular sinus
  • (4) intermediate sinus
  • (5) medullary cords
  • (6) medullary sinus
  • (7) trabecula

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Lymph node: Medulla

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Figure A

  • Medullary cord of lymphoid nodule
  • In addition to lymphocytes, this area contains many plasma cells (ARROWS)

Figure B

  • Label 1: Medullary sinus
  • Label 2: Medullary cord
  • H & E Stain
  • High magnification

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Lymph node: Medulla

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Figure A

  • A photomicrograph of the medulla of a lymph node
  • The medullary sinuses are separated by medullary cords
  • Lymphocytes clearly predominate in number over other cell types
  • A blood vessel within a medullary cord is also seen
  • H & E Stain
  • Medium magnification

Figure B

  • (1) Medullary cords
  • (2) Medullary sinuses

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Lymphoid nodule of the spleen

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  • Lymphoid nodule of the spleen
  • Surrounded by red pulp
  • Label 1: A germinating center
  • Label 2: The (eccentric) central artery
  • Which are characteristics of the spleen & are clearly visible
  • 2 small sections of sheathed arteries are seen to the right of the nodule
  • H & E stain
  • Medium magnification

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Bone marrow: normal

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  • This is the appearance of a normal bone marrow
  • Note the presence of:
  1. megakaryocytes
  2. erythroid islands
  3. granulocytic precursors
  • high magnification

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Bone marrow smear: Normal

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  • This is the appearance of a normal bone marrow smear
  • Note the presence of:
  1. megakaryocytes
  2. erythroid precursors
  3. granulocytic precursors
  • High magnification

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Spleen: Section

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  • The capsule is seen sending the trabeculae to the interior of the organ
  • The red pulp occupies most of the microscopic field
  • Note the white pulp with its arterioles
  • Pirosirius stain
  • Low magnification

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Spleen: Section

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Figure A

  • Section of spleen showing the red pulp (most of microscopic field) and a component of the white pulp – a periarterial lymphatic sheath (PALS) surrounding a central artery
  • Small sections of trabeculae are present in the red pulp
  • H & E stain
  • Low magnification

Figure B

  • (1) PALS
  • (2) central artery
  • (3) red pulp
  • (4) sinusoids

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Blood smear

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  • The RBCs here are normal
  • Have a zone of central pallor about 1/3 size of the RBC
  • The RBCs demonstrate minimal variation is size (anisocytosis) and shape (poikilocytosis)
  • In the center of the field: A band of neutrophil (LEFT) and segmented n
    eutrophil (RIGHT)

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Blood smear

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  • In the center of the field: eosinophil with a bilobed nucleus & numerous reddish granules in the cytoplasm.
  • Just underneath eosinophil: small lymphocyte
  • Eosinophils can increase with: allergic reactions & parasitic infestations

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Blood smear

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  • In the center of the field: basophil with a lobed nucleus (like PMNs) & numerous coarse, dark blue granules in the cytoplasm
  • Infrequent in a normal peripheral blood smear
  • Significance is uncertain
  • Left: Band neutrophil
  • Right: Large activated lymphocyte

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